Journal: Journal of Orthopaedic Translation

Article Title: Targeting FGFR3 signaling and drug repurposing for the treatment of SLC26A2-related chondrodysplasia in mouse model

doi: 10.1016/j.jot.2023.09.003

Figure Lengend Snippet: Inhibition of Fgfr3 in chondrocytes ameliorates chondrocyte proliferation apoptosis and differentiation impairment in mouse growth plate chondrocytes due to Slc26a2 deficiency (A) (B) Ki67 immunostaining and TUNEL staining (red) of tibial sections of P0 mice. Scale bar: 50 ​μm (C) (D) Immunofluorescent analyses of FGFR3 signaling downstream mediators p-ERK1/2 and p-STAT1 (red) in proliferating chondrocytes of tibia sections of P0 mice. Scale bar: 50 ​μm (E) Immunostaining of Col X (red) of tibial sections of P0 mice (F) Quantification of Ki67-labeled cells, TUNEL-labeled cells, and p-ERK1/2- and p-STAT1-positive cells in the growth plate (n ​= ​9/group). For all the above-mentioned statistical analyses, significance was determined by One-way ANOVA followed by Tukey's multiple comparisons test, and the results are shown as the mean ​± ​SD, ∗ P ＜0.05, ∗∗ P ＜0.01. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: The following primary antibodies used were Ki67 (Millipore, Burlington, MA, USA), anti-Col X (Abclonal, A11645, 1:100), anti-phosphatase-ERK1/2 (Cell Signaling Technology, 4370, 1:100), anti-phosphatase-STAT1 (Cell Signaling Technology, 9172, 1:100), anti-SOX9 (Abcam, ab185966, 1:100), anti-Col2α1 (Thermo Fisher, PA1-26206, 1:100), and anti-aggrecan (Thermo Fisher, MA3-16,888, 1:100).

Techniques: Inhibition, Immunostaining, TUNEL Assay, Staining, Labeling

Journal: Journal of Orthopaedic Translation

Article Title: Targeting FGFR3 signaling and drug repurposing for the treatment of SLC26A2-related chondrodysplasia in mouse model

doi: 10.1016/j.jot.2023.09.003

Figure Lengend Snippet: NVP-BGJ398 pharmacological intervention inhibits overactivation of the FGFR3 signaling pathway in Slc26a2 cKO mice (A) Safranin O and fast green staining of sections of tibial growth plates at P49. Slightly increased proliferating and hypertrophic chondrocytes were observed in NVP-BGJ398-treated growth plates compared with those of tamoxifen-induced chondrodysplasia groups. Scale bar: 100 ​μm (B) (C) Ki67 immunostaining and TUNEL staining (red) of tibia sections on P49. Scale bar: 50 ​μm (D) (E) Immunofluorescent analyses of FGFR3 signaling downstream mediators p-ERK1/2 and p-STAT1 (red) in growth plate chondrocytes on tibia sections at P49. Scale bar: 50 ​μm (F) Immunostaining of Col X (red) on tibia sections (G) Quantification of Ki67-labeled cells, TUNEL-labeled cells, and p-ERK1/2- and p-STAT1-positive cells in the growth plate (n ​= ​7). For all the above-mentioned statistical analyses, significance was determined by One-way ANOVA followed by Tukey's multiple comparisons test, and the results are shown as the mean ​± ​SD, ∗P＜0.05, ∗∗P＜0.01, ns: not statistically significant.

Article Snippet: The following primary antibodies used were Ki67 (Millipore, Burlington, MA, USA), anti-Col X (Abclonal, A11645, 1:100), anti-phosphatase-ERK1/2 (Cell Signaling Technology, 4370, 1:100), anti-phosphatase-STAT1 (Cell Signaling Technology, 9172, 1:100), anti-SOX9 (Abcam, ab185966, 1:100), anti-Col2α1 (Thermo Fisher, PA1-26206, 1:100), and anti-aggrecan (Thermo Fisher, MA3-16,888, 1:100).

Techniques: Staining, Immunostaining, TUNEL Assay, Labeling